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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis

Fig. 4

Generation and hepatotoxicity assay of hepatocyte spheroids from iPSCs derived from RA patients. a Scheme of generation of 3D hepatocyte spheroids and morphology of attached spheroids. b Morphology of hepatocyte spheroids generated from HC- and RA-iPSCs. c z-stack confocal microscope image of hepatocyte spheroids expressing albumin and A1AT. d The fluorescence quantity per area of the spheroid calculated by image J program. e 3D spheroid cell viability. HC: healthy control group (n = 3), blue bar; RA: RA patient group (n = 3), red bar. f Cell viability data relative to vehicle (MTX, 0 nM). HC: healthy control group (n = 3), blue line; RA: RA patient group (n = 3), red line. Statistical significance is expressed as p value (Student’s t test) vs. 0 nM: *p < 0.05; **p < 0.01; ***p < 0.001. g Live/dead cell staining. Each image was obtained from a confocal z-stack. h Mitotracker and Hoechst staining, obtained from confocal z-stacks

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