Table 1 Sequences of primers used for PCR
Target name | Direction | Primer sequence (5′–3′) | Size (bp) |
|---|---|---|---|
OCT3/4 | Forward | ACCCCTGGTGCCGTGAA | 190 |
Reverse | GGCTGAATACCTTCCCAAATA | ||
SOX2 | Forward | CAGCGCATGGACAGTTAC | 321 |
Reverse | GGAGTGGGAGGAAGAGGT | ||
NANOG | Forward | AAAGGCAAACAACCCACT | 270 |
Reverse | GCTATTCTTCGGCCAGTT | ||
DPPA5 | Forward | CGGCTGCTGAAAGCCATTTT | 215 |
Reverse | AGTTTGAGCATCCCTCGCTC | ||
LIN28 | Forward | GTTCGGCTTCCTGTCCAT | 122 |
Reverse | CTGCCTCACCCTCCTTCA | ||
ALB | Forward | TTGGCACAATGAAGTGGGTA | 161 |
Reverse | AAAGGCAATCAACACCAAGG | ||
CYP1A2 | Forward | ATGGCATTGTCCCAGTCTGTT | 135 |
Reverse | TGGCTCTGGTGGACTTTTCAG | ||
CYP2D6 | Forward | GTGTCCAACAGGAGATCGACG | 101 |
Reverse | CACCTCATGAATCACGGCAGT | ||
CYP2E1 | Forward | GTTCTTTGCGGGGACAGAGA | 202 |
Reverse | GAGGGTGATGAACCGCTGAA | ||
CYP3A4 | Forward | TGTGCCTGAGAACACCAGAG | 226 |
Reverse | GTGGTGGAAATAGTCCCGTG | ||
CYP3A7 | Forward | GAAACACAGATCCCCCTGAA | 105 |
Reverse | TCAGGCTCCACTTACGGTCT | ||
UGT1A1 | Forward | CAGCAGAGGGGACATGAAAT | 174 |
Reverse | ACGCTGCAGGAAAGAATCAT | ||
UGT2B15 | Forward | GTGTTGGGAATATTATGACTACAGTAAC | 157 |
Reverse | GGGTATGTTAAATAGTTCAGCCAGT | ||
OATP1B1 | Forward | GAGCAACAGTATGGTCAGCCT | 135 |
Reverse | GGCAATTCCAACGGTGTTCA | ||
OATP1B3 | Forward | GTCCAGTCATTGGCTTTGCA | 111 |
Reverse | CAACCCAACGAGAGTCCTTAGG | ||
NTCP | Forward | GGGACATGAACCTCAGCATT | 199 |
Reverse | CGTTTGGATTTGAGGACGAT | ||
MRP2 | Forward | AGCGTCCTCTGACACTCG | 206 |
Reverse | GGCATCTTGGCTTTGACT | ||
MDR1 | Forward | CTAATGCCGAACACATTGGA | 237 |
Reverse | CAGTCGCTTTATTTCTTTGCC | ||
AHR | Forward | CAAATCCTTCCAAGCGGCATA | 123 |
Reverse | CGCTGAGCCTAAGAACTGAAAG | ||
FXR | Forward | CAGGATTTCAGACTTTGGACCAT | 63 |
Reverse | CTTCAACCGCAGACCCTTTC | ||
GR | Forward | ATAGCTCTGTTCCAGACTCAACT | 111 |
Reverse | TCCTGAAACCTGGTATTGCCT | ||
PPARα | Forward | AGAGATTTCGCAATCCATCGG | 62 |
Reverse | ACTGGTATTCCGTAAAGCCAAAG | ||
RXRA | Forward | ATGGACACCAAACATTTCCTGC | 211 |
Reverse | GGGAGCTGATGACCGAGAAAG | ||
SHP | Forward | CCCCAAGGAATATGCCTGCC | 233 |
Reverse | TAGGGCGAAAGAAGAGGTCCC | ||
ADORA1 | Forward | GTCCTCATCCTCACCCAGAG | 189 |
Reverse | CAGATTGTTCCAGCCAAACA | ||
ADORA2A | Forward | CGAGGGCTAAGGGCATCATTG | 98 |
Reverse | CTCCTTTGGCTGACCGCAGTT | ||
ADORA2B | Forward | CTCTTCCTCGCCTGCTTCGTG | 107 |
Reverse | TTATACCTGAGCGGGACACAG | ||
ADORA3 | Forward | TACATCATTCGGAACAAACTC | 80 |
Reverse | GTCTTGAACTCCCGTCCATAA | ||
CD39 | Forward | ACAGGCGTGGTGCATCAAGTAGAA | 279 |
Reverse | CCTGGCACCCTGGAAGTCAAAG | ||
CD73 | Forward | CAGTACCAGGGCACTATCTG | 194 |
Reverse | AGTGGCCCCTTTGCTTTAAT | ||
GAPDH | Forward | GAATGGGCAGCCGTTAGGAA | 414 |
Reverse | GACTCCACGACGTACTCAGC |