Fig. 4

IFNγ/TNFα sensitizes BM-MSCs to Fas-mediated apoptosis iNOS-dependently. a Wild-type, Fas^−/−, and iNOS^−/− BM-MSCs were treated with IFNγ/TNFα for 24 h or for the indicated time. The levels of Fas expression were quantified by real-time PCR. b, c Wild-type, and iNOS^−/−, BM-MSCs were treated with the indicated conditions for 48 h. Apoptotic cells were analyzed using flow cytometry. The percentage of Annexin V-positive cells relative to untreated controls is indicated in a bar chart. *P < 0.05, **P < 0.01, ***P < 0.001