Fig. 5

Blockade of autophagy by iNOS sensitizes BM-MSCs to apoptosis. a Apoptotic cells were analyzed using flow cytometry in MSCs treated or not (ctrl) for 48 h with IFNγ/TNFα (10 ng/ml each), alone or in combination with chloroquine (CQ), rapamycin. The percentage of Annexin V-positive cells relative to untreated controls is indicated in a bar chart. b Representative Western blot of LC3I/LC3II, p62, PARP, and Caspase-3 from wild-type BM-MSCs and iNOS^−/− BM-MSCs pretreated or not with IFNγ/TNFα (10 ng/ml each) for 16 h and treated with rapamycin for the indicated time