Table 1 Gene-specific primers for qRT-PCR
Genes | Oligonucleotide sequence (5′–3′) |
|---|---|
β-actin | F: CAACGAGCGGTTCCGATG |
R: GCCACAGGATTCCATACCCA | |
Atf4 | F: CGGGTGTCCCTTTCCTCTTC |
R: TGAAGAGCGCCATGGCTTAG | |
Bip/GRP78 | F: GTGTGTGAGACCAGAACCGT |
R: ACAGTGAACTTCATCATGCCG | |
Erdj4/Dnajb9 | F: GGGGCGCACAGGTTATTAGAA |
R: TCTGAGGCAGACTTTGGCAC | |
Chop | F: GGAACCTGAGGAGAGAGTGTT |
R: AAGGTGAAAGGCAGGGACTC | |
Xbp1 | F: GCAGCAAGTGGTGGATTTGG |
R: CCTTACTCCACTCCCCTTGG | |
Fas | F: GCTTGCTGGCTCACAGTTAAG |
R: AGGTTGGTGTACCCCCATTC | |
Bcl2 | F: CACCCCTGGTGGACAACATC |
R: ATAGTTCCACAAAGGCATCCCAG | |
Bcl2l11 | F: GCCAGGCCTTCAACCACTAT |
R: TGCAAACACCCTCCTTGTGT | |
Bax | F: TGCTAGCAAACTGGTGCTCA |
R: AGTAGGAGAGGAGGCCCAGC |