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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Comparative analysis of mesenchymal stem cells derived from amniotic membrane, umbilical cord, and chorionic plate under serum-free condition

Fig. 3

Osteogenesis of AM-MSCs, UC-MSCs, and CP-MSCs. a–c Representative pictures of three MSCs before and after the osteogenic differentiation were shown. The differentiation results of AM-MSCs (a), UC-MSCs (b), and CP-MSCs (c) were confirmed by Alizarin Red staining. Scale bar = 200 μm. d–f Real-time PCR was performed to measure the relative expression levels of osteogenic differentiation related genes: ON (d), OCN (e), and RUNX2 (f). All data were presented as the mean ± SEM (n = 3). Each sample was replicated in triplicates. *p < 0.05; ***p < 0.001

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