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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Development of immortalized Hertwig’s epithelial root sheath cell lines for cementum and dentin regeneration

Fig. 2

HERS-C2 and HERS-H1 underwent EMT induced by TGF-β1. a With the control culture media, HERS-C2 and HERS-H1 showed the cubostone-like morphology of epithelial cells. After 7 days induction by TGF-β1, HERS-C2 and HERS-H1 became elongated. Scale bars: 50 μm. b, c Expression of EMT markers, such as E-cadherin, Vimentin, N-cadherin, Twist1, Snail1, and Zeb1 was examined by real-time RT-PCR in b HERS-C2 cells and c HERS-H1 cells after 3 days treatment with TGF-β1. (*P < 0.05; **P < 0.01; ***P < 0.001 vs. control)

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