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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Development of immortalized Hertwig’s epithelial root sheath cell lines for cementum and dentin regeneration

Fig. 3

Cementogenetic differentiation potential of the two cell lines. a, b The expression of cementoblasts markers was examined by real-time RT-PCR in the primary, HERS-H1, and HERS-C2 cells after 3 and 7 days culture of osteo-medium and combined treatment with TGF-β1 and osteo-medium. c Representative images of Alizarin Red S staining indicated the potential of cemento-differentiation of HERS-H1 and HERS-C2 cells. d, e, f Quantitative analyses of calcium mineralization in d HERS-C2 cells and f HERS-H1 cells; f comparison between HERS-H1 and HERS-C2 cells. (*P < 0.05 vs. control group in a, b, d, e; #P < 0.05 vs. osteo-induction group; *P < 0.05 vs. HERS-H1 in f)

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