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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification

Fig. 2

In vivo clonal analysis of subpopulations in the MSC niche in triple transgenic mice. a, b Typical images from early (a) and late (b) lesions of Nse-BMP4;Tie2-cre;R26R-Confetti mice. Note that many clonal clusters are located in the proposed MSC niche in (b) (between the white dashed lines). c, d Typical images from early (c) and late (d) lesions of Nse-BMP4;Gli1-creERT;R26R-Confetti mice. Note that the labeling efficiency was generally low in Gli1-creERT;R26R-Confetti mice, and the clonal clusters were easily identifiable. Similarly, the majority of clonal clusters were located in the proposed MSC niche (between the white dashed lines). e, f Typical images from early and late lesions of Nse-BMP4;Gli1-creERT;R26R-Confetti mice. Similarly, the majority of clonal clusters were located in the proposed MSC niche. White arrows point to clonal clusters in all panels. g Summary of in vivo clonal analysis in developing HO in each triple transgenic mice, n = 5 / group. h Typical image from late stages (3 weeks after injury) of lesions that appear to catch the migration pattern of progeny (white arrows, from MSC to inner core). a, d, g, and j are on the same scale; b, e, h, and k are on the same scale, bar = 50 μm. n and o are on the same scale, af are on the same scale; bar = 50 μm.

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