Table 1 Primer sequences and condition for qRT-PCR
Gene | Primer nucleotide sequence | Product size (bp) | Annealing temperature | Accession no |
|---|---|---|---|---|
GAPDH | 5′-CGGCAAGTTCAACGGCACAG-3′ (forward) 5′-GAAGACGCCAGTAGACTCCACGAC-3′ (reverse) | 148 | 60 | NM_017008.4 |
BMP2 | 5′-CCCTTTGTATGTGGACTTCAGTGATGTG-3′ (forward) 5′-CTATGGCATGGTTGGTGGAGTTCAG-3′ (reverse) | 137 | 63 | NM_017178.1 |
OPN | 5′-CAGTCGATGTCCCTGACGG-3′ (forward) 5′-GTTGCTGTCCTGATCAGAGG-3′ (reverse) | 206 | 60 | NM_012881.2 |
OCN | 5′-GGTGCAAAGCCCAGCGACTCT-3′ (forward) 5′-GGAAGCCAATGTGGTCCGCTA-3′ (reverse) | 199 | 60 | NM_013414.1 |
ALP | 5′-ACCATTCCCACGTCTTCACATTT-3′ (forward) 5′-AGACATTCTCTCGTTCACCGCC-3′ (reverse) | 162 | 60 | NM_013059.1 |
Col I | 5′-CATCGGTGGTACTAAC-3′ (forward) 5′-CTGGATCATATTGCACA-3′ (reverse) | 238 | 55 | NM_053356.1 |
TNMD | 5′-CCATGCTGGATGAGAGAGGTTAC-3′ (forward) 5′-CACAGACCCTGCGGCAGTA-3′ (reverse) | 72 | 58 | NM_022290.1 |
Scx | 5′-AACACGGCCTTCACTGCGCTG-3′ (forward) 5′-CAGTAGCACGTTGCCCAGGTG-3′ (reverse) | 102 | 58 | NM_001130508.1 |
Col II | 5′-GAGTGGAAGAGCGGAGACTACTG-3′ (forward) 5′-CTCCATGTTGCAGAAGACTTTCA-3′ (reverse) | 81 | 55 | NM_012929.1 |
Sox9 | 5′-CTGAAGGGCTAGGACTGGAC-3′ (forward) 5′-TACTGGTCTGCCAGCTTCCT-3′ (reverse) | 140 | 58 | NM_080403.1 |