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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Sirt6 regulates efficiency of mouse somatic reprogramming and maintenance of pluripotency

Fig. 4

Sirt6-null iPS-like cell line has differentiation defect. a EB formation of different time points in Sirt6 knockout iPS-like cells compared to wild-type control. b Pluripotency genes (Nanog, Oct4, Esrrb) in Sirt6-null iPS-like cells were analyzed compared to wild-type iPSCs by qRT-PCR during EB formation. Data were normalized to the expression levels in ESCs. Data represented as mean ± SD from three independent assays. c Western blot analysis showed the expression of Oct4 and Sox2 in Sirt6-null iPS-like cells compared to wild-type iPSCs in EB differentiation. d GFP-positive cell retained more percentage in Sirt6-null iPS-like cells on OP9 co-cultured hematopoietic differentiation system, 50×. e c-kit + cells percentage by FACS in day9 on OP9 co-cultured hematopoietic differentiation from wild-type iPS cells and Sirt6-null iPS-like cells. f c-Kit and Sca1 gene expression levels were analyzed by qRT-PCR in wild-type iPS cells and Sirt6-null iPS-like cells induced hematopoietic progenitors at day 9 on OP9 co-cultured hematopoietic differentiation system. Data were normalized to the expression levels in wild-type control. Data represented as mean ± SD from three independent assays

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