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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Sirt6 regulates efficiency of mouse somatic reprogramming and maintenance of pluripotency

Fig. 5

Transcriptome analysis identifies differential expressed genes in Sirt6-null iPS-like cells and Sirt6-null MEFs. a Transcriptome analysis in wild-type iPS cell line and Sirt6-null iPS-like cell. Each dot represents an individual gene; differentially expressed genes are depicted according to FPM (fragments per million mapped reads) values. Red dots represent the 63 significantly upregulated genes, and blue dots represent the 144 significantly downregulated genes. Some pluripotency-related genes like Nr5a2, Sall1, Fbx15, Foxd3, and Tcf15 were highlighted and arrowed. b Enriched Gene Oncology (GO) terms of the upregulated gene lists in Sirt6-null iPS cell line. c Transcriptome analysis in wild-type MEF and Sirt6-null MEF. Each dot represents an individual gene; differentially expressed genes are depicted according to microarray probe signal values. Red dots represent the 325 significantly upregulated genes, and blue dots represent the 457 significantly downregulated genes. Some pluripotency-related genes like Nr5a2, Sall1, Fbx15, Foxd3, and Tcf15 were highlighted and arrowed. d Enriched Gene Oncology (GO) terms of the upregulated gene lists in Sirt6-null MEFs. e Venn diagrams showing the overlap of upregulated gene lists between Sirt6-null iPS-like cell line and Sirt6-null MEFs. f Real-time qPCR analysis of gene expression including Nr5a2, Sall1, Sall3, FbXO15, Zfp42, Foxd3, and Tcf15. Data were normalized to the expression levels in wild-type iPSCs. Data represented as mean ± SD from three independent assays

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