Fig. 4

Localization of engrafted MSCs in infarcted hearts. a Heart schematic pinpointing the location of ligation (cross), injection (arrow), and sampling (dashed line). b Engraftment of genetically modified MSCs indicated by co-localization of mCherry fluorescence (red) and immunofluorescent signal against mCherry (green) on post-injection 1 day. Scale bar = 50 μm. c Quantification of cell engraftment by mCherry fluorescence (red) (n = 3–4). d Representative images of surviving MSCs indicated by CM-Dil staining on post-therapy 3 and 7 days. Scale bar = 200 μm. e Quantification of cell engraftment by CM-Dil signal (n = 5–10). *P < 0.05. CM-Dil, chloromethylbenzamido