Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Involvement of tumor necrosis factor alpha in steroid-associated osteonecrosis of the femoral head: friend or foe?

Fig. 1

The content of TNFα in normal tissues and necrotic tissues in patients with steroid-associated ONFH. a The normal tissues (NT) and osteonecrotic tissues (ONT) were obtained. b, c Hematoxylin and eosin staining of the tissues, typical sign of empty lacunae was observed in ONT (magnification, × 200). d Relative levels of TNFα in normal tissues and necrotic tissues. The content of TNFα was measured by ELISA assay and normalized with total proteins. Data is presented as mean ± SD (n = 60, *p < 0.05). e, f Detection of TNFα in bone samples by immunohistochemical staining. Bone samples of NT and ONT were decalcified and sectioned. Anti-TNFα antibody was used for immunohistochemical staining (magnification, × 100)

Back to article page