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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Involvement of tumor necrosis factor alpha in steroid-associated osteonecrosis of the femoral head: friend or foe?

Fig. 5

ChIP analysis of H3K4me3 and H3K27me3 in rat Runx2 promoter. a Decreased occupancy of H3K4me3 and b increased occupancy of H3K27me3 on rat Runx2 promoter in rMSCs treated with TNFα. The histone modifications of rat Runx2 promoter were analyzed by the ChIP-PCR assay. ChIP was done using anti-H3K4me3 and anti-H3K27me3 monoclonal antibodies, and PCR was performed with specific primers. Normal rabbit IgG was used as loading control. Values are expressed as mean ± SD of three independent experiments. ##p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512