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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Sex influences DNA methylation and gene expression in human skeletal muscle myoblasts and myotubes

Fig. 3

Genes with both differential DNA methylation and gene expression in human myoblasts from women versus men. ad Bar graphs showing mRNA expression and tables showing DNA methylation of a, b XIST and c, d LAMP2 and RPS4X in myoblasts from women versus men. Only data for CpG sites with significant differences between women and men are included. Below the tables are UCSC genome browser tracks for b XIST and d LAMP2 showing the location of the promoter region, CpG sites in the table above, the Refseq gene and histone modifications from ENCODE data in skeletal muscle myoblasts (HSMM). Red line on the chromosome above indicates location on the X-chromosome. N = 11–13, *q < 0.05; H3K4me1, Histone 3 lysine 4 (H3K4) methylation; H3K4me3, H3K4 trimethylation; H3K27ac, H3K27 acetylation; TSS200/TSS1500, 0–200 bp and 200–1500 bp upstream of the transcription start site (TSS), respectively. e In vitro methylation of LAMP2 and RPS4X promoters reduced expression (activity) of a luciferase reporter gene. Numbers above the bars indicate the number of cytosines targeted by each enzyme in the promoter sequences (SssI: all CG, HhaI: GCGC and HpaII: CCGG). *p < 0.05, N = 5–6. Mean of the control for each gene was set to 1. Data are presented as mean ± SD

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