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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: OCT4 maintains self-renewal and reverses senescence in human hair follicle mesenchymal stem cells through the downregulation of p21 by DNA methyltransferases

Fig. 1

The validation of the fluorescence efficiency and the expression of OCT4 in transduced hHFMSCs. a The cell morphologies of transduced hHFMSCs (hHFMSCsEGFP and hHFMSCsOCT4) were changed between 0 and 10 days after OCT4 transduction. b Flow cytometry assay of GFP expression in hHFMSCs after 12 days of transduction. Live cells were identified by 7AAD exclusion. qPCR (c), western blot (d), and flow cytometry (e) results for the expression of OCT4 in hHFMSCsEGFP and hHFMSCsOCT4. f Immunofluorescence of OCT4 expression and location in hHFMSCsEGFP and hHFMSCsOCT4 (bar, 20 μm) (EGFP, hHFMSCsEGFP; OCT4, hHFMSCsOCT4; **p < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512