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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: OCT4 maintains self-renewal and reverses senescence in human hair follicle mesenchymal stem cells through the downregulation of p21 by DNA methyltransferases

Fig. 7

DNMT inhibition decreased OCT4-increased proliferation capacity and differentiation potential in hHFMSCs. a The impact of 5-aza-dC (100 μM, 72 h) and zebularine (100 μM, 48 h) treatment on the expression of DNMTs, p21, OCT4, PCNA, and cyclin D1. b Immunofluorescence of proliferation-associated protein Ki67 (left panels) and OCT4 (right panels) expression and location after DNMT inhibition (bar, 50 μm). c MSP of p21 in hHFMSCsOCT4 treated with DMSO, 5-aza-dC, or zebularine. Cell proliferation curve (d) and clone formation assay (e) after DNMT inhibition; and the enlarged views showed the difference between DMSO and DNMT inhibitor-treated hHFMSCsOCT4. The histogram is the formation rate of the three clones. f Effects of DNMT inhibition on hHFMSC cell cycle phase distribution. The percentage of the G1, G2, and S phases in the cell cycle (left of the lower panel) and the PI (right of the lower panel) after DNMT inhibition. “dip” is the abbreviation for diploid. g Osteogenic differentiation after DNMT inhibition. Calcium nodules were detected by Alizarin Red S staining (bar, 50 μm). (*p < 0.05; **p < 0.01)

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