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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: OCT4 maintains self-renewal and reverses senescence in human hair follicle mesenchymal stem cells through the downregulation of p21 by DNA methyltransferases

Fig. 8

DNMT inhibition promoted senescence in hHFMSCs. a qPCR results for the expression of senescence-associated genes. b Effects of DNMT inhibition on hHFMSCs under transmission electron microscopy. (I) Rich microvilli in the cell surface, regularly shaped nuclei, and abundant euchromatin in the nucleus. (II) Normal mitochondria. (III) The cytoplasm rich in rough endoplasmic reticulum. (IV) Some cells containing lipid drops. (V, IX) The microvilli in the cell surface were almost completely removed, and some of the nuclei are irregular in shape. (VI, X) The dilation of the rough endoplasmic reticulum (white arrow) and the turgidity and vacuolization of the mitochondrion (black arrow). (VII, XI) The secondary lysosomes (black arrow) and lipid drops (white arrow) in some cells. (VIII, XII) The contraction of the Golgi body. c SA-β-gal staining after DNMT inhibition (bar, 50 μm); stained cells are indicated by arrows. (*p < 0.05; **p < 0.01)

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