Table 1 Primers for qPCR, ChIP, and MSP
Gene | Forward primers (5′ to 3′) | Reverse primers (5′ to 3′) |
|---|---|---|
OCT4 | CTGAAGCAGAAGAGGATCAC | GACCACATCCTTCTCGAGCC |
p21 | TGGAGACTCTCAGGGTCGAAA | GGCGTTTGGAGTAGAAATC |
p16 | GTGGACCTGGCTGAGGAG | CTTTCAATCGGGGATGTCTG |
hTERT | CATGGGCACGTCCGCAA | GGCGTGGTGGCACATGAA |
DNMT1 | ACCGCTTCTACTTCCTCGAGGCCTA | GTTGCAGTCCTCTGTGAACACTGTGG |
DNMT3a | CACACAGAAGCATATCCAGGAGTG | AGTGGACTGGGAAACCAAATACCC |
DNMT3b | AATGTGAATCCAGCCAGGAAAGGC | ACTGGATTACACTCCAGGAACCGT |
GAPDH | CCATGTTCGTCATGGGTGTGA | CATGGACTGTGGTCATGAGT |
DNMT1 (ChIP) | CCCCACACACTGGGTATAGAA | CGAGGCATTCATTCATTCATT |
DNMT3a (ChIP) | TCATTTCATTCAACCTCCCAC | CTCTGAGATGAGCTGCCTTGA |
DNMT3b (ChIP) | CATCCAAAGAATTAACCCTTCACT | GGTCTCCAAGTGCTTATAGTGGAT |
p21 U (MSP) | TTTTGGGATTGGTTGGTTTG | ACACCCAACTCCAACTCCAC |
p21 M (MSP) | TTGTAGTACGCGAGGTTTCG | CAACTCAACGCGACCCTAAT |