Fig. 2

Assessment of Nanomag uptake, cell morphology and Nanomag properties as a consequence of GET complexing. Prussian blue staining highlights the presence of iron-based magnetic nanoparticles by blue staining. Ai Prussian blue staining of oMSCs incubated with no SPIONs, Nanomag only and GET-Nanomag. Aii Prussian blue staining of GET-Nanomag-labelled oMSCs demonstrating similar uptake by all cells within a single culture well. B Prussian blue staining of GET-Nanomag-labelled MSCs from six sheep donors demonstrate consistent uptake across multiple donors. Ci Zetapotential measurements of Nanomag and GET-Nanomag determined in water. Cii Hydrodynamic diameter of Nanomag and GET-Nanomag determined in water. Data in Ci and Cii represents the mean zeta potential (ζ) ± s.d (n = 3) and hydrodynamic diameter ± s.d (n = 3) respectively with significance determined by unpaired t-test where **** is p < 0.0001. Scale bars = 100 μm