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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: DP71 and SERCA2 alteration in human neurons of a Duchenne muscular dystrophy patient

Fig. 2

Confocal immunofluorescence reaction of TuJ-1 in CTRL (a, b) and DMD (c) neurons reveals prevalently multipolar control neurons (b inset) and unipolar and bipolar DMD neurons (c). VGLUT-1(green) and NFH (red) dual immunofluorescence shows DMD abnormal neurons with spread out processes (hm, arrows) compared with the control ones (dg). Neurons of both CTRL and DMD (d, h) display an orange fluorescence signal of VGLUT and NFH colocalization. Ultrastructural analysis shows DMD neurons with dilated processes containing autophagic vacuoles (n arrow), differently to healthy control neurons (o). Scale bar: a 40 μm; b 60 μm; c 20 μm; d, g, h, m, 10 μm; e, f, i, l 20 μm; n, o 0.5 μm

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