Fig. 3
From: DP71 and SERCA2 alteration in human neurons of a Duchenne muscular dystrophy patient
a–f The Dp71 (green) and AQP4 (red) confocal analysis shows the reduction of Dp71 fluorescence in DMD-neurons (d, e) compared with a strong Dp71 signal in the control ones (a, b) while the AQP4 fluorescence is missing in DMD neurons (d, f) and weakly expressed in the control (a, c). g–n Dys (red) and βDG (green) dual reaction shows a lower βDG signal in DMD neurons (l, n) compared to the control ones (g, i) and a Dys clustering in DMD cytoplasm (l and inset arrow, m), and a diffused signal in the control cytoplasm (g and inset arrow, h). o Morphometric analysis shows a significant reduction of Dp71 and Dys fluorescence intensity in DMD neurons compared to control. p The mRNA expression analysis shows reduction of Dp71, AQP4 mRNA, and an increase of DG and Dys mRNA in DMD neurons compared to control. Scale bar: a–f 12 μm; g–n 20 μm. Data are represented as mean ± SEM