Fig. 1
Experimental design for mouse models of acute liver injury and chronic hepatic fibrosis. Eight weeks of BALB/C nude mice were used in both acute and chronic liver injury models. a In acute liver injury model, single CCl4 dissolved in olive oil (0.5 μl/g) were administrated by intra-peritoneal injection. Intra-splenic iHEPs transplantation was processed at 24 h after administration of CCl4. Mice were sacrificed at specific time points. b In chronic hepatic fibrosis model, CCl4 (0.25 μl/g) or olive oil was intra-peritoneally injected twice a week during 8 weeks. In CCl4 + iHEPs group, iHEPs (1 × 106 cells/100 μl, either type A or type B) were transplanted into spleen at the end of 8 weeks of CCl4 injections. After a total of 10 weeks of CCl4 treatment, all mice were sacrificed for analysis. c Immunofluoresence staining for characterization of induced hepatocyte-like cells (types A and type B) DAPI, nuclear staining; 488-Green, anti-albumin staining, 594-Red, anti-CK18; scale bar, 100 μm