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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: The therapeutic potential of induced hepatocyte-like cells generated by direct reprogramming on hepatic fibrosis

Fig. 3

Identification of migration and function of intra-splenically transplanted iHEPs in acute liver injury model. The migration of intra-splenically transplanted iHEPs into the liver was investigated using alpha-GFP-labeled iHEPs. a Immunofluorescence staining of liver after 4 h of DMEM intra-splenic injection. b iHEPs intra-splenic transplantation. DAPI, nuclear staining; 488-Green, GFP-labeled iHEPs-type A fluorescence; 594-Red, anti-GFP immunofluorescence staining; Merge, merged image of 488-Green and 594-Red. Scale bar, a, 50 μm; b, 20 μm. c Upper and lower rows represent immunofluorescence staining in two different sites of liver sections. DAPI, nuclear staining; GFP, GFP fluorescence; Albumin, anti-albumin staining; Merge, merged image of GFP and albumin staining. Scale bar, 20 μm. d Western blotting for GFP or β-actin from mouse liver tissue sacrificed at indicated time points. NC, negative control; PC, positive control (iHEPs)

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Stem Cell Research & Therapy

ISSN: 1757-6512