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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human liver stem cells attenuate concanavalin A-induced acute liver injury by modulating myeloid-derived suppressor cells and CD4+ T cells in mice

Fig. 2

Characterization of HYX1 cells. a During the first 20 days in culture, the cells formed clusters and appeared round. b In subculture, the cells spread out and looked flattened and larger (original magnification × 100). c Representative TEM image of an HYX1 cell. d ICG uptake assay showed pale green ICG-positive cells (as indicated by the yellow arrows). e PAS staining of HYX1 showed positive cells with cytoplasm appeared red (as indicated by the blue arrows). f, g HYX1 cells were treated with DMSO + HGF or PBS (control group) for 5 days and western blot analysis of the indicated proteins. h RT-PCR for the indicated genes. Data are presented as mean ± SEM; compared with control group, *P < 0.05, **P < 0.01, as determined by t test

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