Fig. 5

Migration analysis in an agarose spot assay. A Representative images of eMSC-4C migration assay into a PBS-, TNF-α-, IL-1β- or IFN-τ-containing agarose spot at the indicated times. Images were obtained in a light stereomicroscope at × 20 magnification. b and c Migratory response of MSC to inflammatory and implantational stimuli. b The distance migrated from the border of the PBS-agarose spot was measured in two independent experiments for all eMSC lines at the indicated times (mean ± SD). Different letters indicate significant differences: p < 0.005 for eMSC migration mediated by PBS at day 7 (a, b, c, d, e); C p < 0.05 for eMSC migration mediated by PBS (a, b, c), p < 0.005 for eMSC migration mediated by TNF-α (e, f, g) and p < 0.05 for eMSC migration mediated by IL-1β (i, j, k, l) and IFN-τ (p, q). *p < 0.05; ***p < 0.0005; ****p < 0.0001