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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells

Fig. 1

HSDCs from E12.5 fetal mouse livers contained Dlk1+ and Dlk1 subpopulations. a Representative pictures of fetal livers dissected form E12.5, E13.5, and E14.5 mice (n = 6). b Cells were isolated from fetal livers, and hepatic spheroids were obtained from floating cultures on ultralow attachment plates for 6 days. c E12.5 spheroids were collected from ultralow attachment plates and plated onto collagen I-coated plates. d E12.5 HSCDs were collected, and the expression of AFP, ALB, and CK19 was detected via immunofluorescence. e The immunophenotype of E12.5 HSDCs was assessed with FACS. f The expression of Dlk1 in E12.5 HSDCs was detected by FACS. Representative images from one of three experiments are shown

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