Fig. 2

HSDCs from E12.5 fetal mouse livers possessed multidifferentiation potency. a, b HSDCs were cultured in hepatic differentiation medium for 3 weeks, and then, PAS staining was performed to detect synthesized glycogen (a), and the expression of hepatic markers (ALB, G6P, TO) was measured via RT-PCR (b). c–e HSDCs were induced into cholangiocytes in three-dimensional culture for 8 days, and the formation of cyst (c) and bile duct-like structures is indicated by arrows (d); the expression of the cholangiocytic marker CK19 was measured via RT-PCR (E). f–h HSDCs were cultured in either adipogenic or osteogenic medium. f Oil Red O was used to evaluate adipogenesis, and Alizarin Red S was used to detect osteogenesis. g Expression level of adipocyte marker PPAR-γ2 was assessed by RT-PCR. h The expression level of the osteocyte marker OPN was assessed using RT-PCR. Crtl. control, Diff. differentiated. Representative images from one of three experiments are shown