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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells

Fig. 6

Dlk1M served as a regulator of differentiation by affecting AKT and MAPK signaling. Dlk1 cells were infected with mock- or Dlk1M-expressing viruses, and the expression of Dlk1M was detected by FACS (a) and western blotting (b). c Dlk1 cells infected with mock- or Dlk1M-expressing viruses were cultured under hepatic induction conditions with OSM and Matrigel. The expression levels of ALB, G6P, and TO were detected using quantitative real-time PCR. d Dlk1 cells infected with mock- or Dlk1M-expressing viruses were cultured under cholangiocytic induction conditions. The expression level of CK19 was detected using quantitative real-time PCR. e, f Dlk1 cells infected with mock- or Dlk1M-expressing viruses were cultured for 4 days under normal culture conditions. The expression levels of Notch receptors and ligands were determined by quantitative real-time PCR (e), and signaling pathways were analyzed via western blotting (f). Representative images from one of three experiments are shown, and the data are shown as the mean ± SEM of three independent experiments. *P < 0.05 (Mann-Whitney test in d and two-way ANOVA with post Bonferroni’s multiple comparisons test in c and e)

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