Fig. 3
Effect of SHED on antigen-specific antibody and cytokine production. OVA-specific IgE, IgG1, and IgG2a levels in serum (a) and characteristic cytokines of Th1, Th2, and Th17 cells in lymphocyte culture supernatant (b) were measured by ELISA kits. OVA-specific IgE, IgG1, and IgG2a levels were higher in the OVA group than in the control and sham-SHED groups. In addition, IgE and IgG1 were significantly reduced upon SHED and BMMSC treatments but not IgG2a. Both SHED and BMMSC treatments significantly enhanced the expression of IFN-γ but reduced IL-4, IL-5, IL-13, and IL-17A levels. The downregulation of IL-4 and IL-13 levels in the SHED group was more obvious compared with that in the BMMSC group at the same dose. Data are expressed as the mean ± SD (n = 6 in each group) from three representative experiments. *p < .05, **p < .01, ***p < .001