Fig. 3

Tracking of hAD-MSCs in vivo. a, b PKH26-labeled hAD-MSCs showed red fluorescence in vitro (a × 100) and in vivo (b × 800). c The labeling rate of PKH26-labeled hAD-MSCs was analyzed using flow cytometry. d The growth curves of PKH26-labeled and unlabeled hAD-MSCs were analyzed using a CCK-8 assay (n = 6). e, f Transplanted PKH26-labeled hAD-MSCs were observed at 24 h after cell transplantation in ovaries (e × 100) and other organs (f × 100) of SD rats in the hAD-MSC-treated group. *P < 0.05 and **P < 0.01. Scale bars, 100 μm