Fig. 5

Analysis of direct adipogenesis of EBs derived from pluripotent stem cells. The fluorescence image analysis (top half) and Oil Red O image analysis (bottom half) of the entire well (top row) and of the representative microscopic field (middle rows) are shown along with their histograms (bottom row) for the 3 samples: human iPSC line 1 (a), human iPSC line 2 (b), human HUES6 ESC line (c). Phase contrast images are also shown as references for fluorescent analysis in the top half. The clump of cells to the left in the top row image of (b) are dead or dying, and therefore their nuclei fail to register and are excluded from consideration by the FATS analysis. Red scale bars: 100 μm, yellow scale bars: 1 mm