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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagy

Fig. 1

Characterization of MSCs and the effects of autophagy agonist and inhibitor. The MSCs were cultured in basic medium for 10 days before analyses. a Representative image showing second generation MSCs on day 10 of culture; scale bars = 50 μm. Flow cytometry histograms showing percentage of MSC marker-positive cells (red curve); purple shaded area represents the control. b Immunoblots showing the levels of Beclin1, LC3I, and LC3II in MSCs, with β-actin as the loading control. c Representative electron microscopy images showing autophagosomes (arrows); scale bars = 0.5 μm. d Left panel—representative immunofluorescence images showing LC3+ cells (green); scale bars = 50 μm. Right panel—bar graph comparing the number of LC3+ cells. The values are the mean ± SD of triplicate experiments; *P < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512