Fig. 2From: Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagyEffects of LIPUS on autophagy in MSCs. The MSCs were cultured in basic medium or chondrogenic medium for 10 days before analyses. a Immunoblots showing the levels of Beclin1, LC3I, and LC3II in differentiating MSCs stimulated with varying intensities of LIPUS, with β-actin as the loading control. b Representative electron microscopy images showing autophagosomes (arrows); scale bars = 0.5 μm. c Left panel—representative immunofluorescence images showing LC3+ cells (green); scale bars = 50 μm. Right panel—bar graph comparing the number of LC3+ cells. The values are the mean ± SD of triplicate experiments; *P < 0.05Back to article page