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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagy

Fig. 4

Effects of LIPUS on autophagy in MSCs treated with autophagy agonist or inhibitor. The MSCs were cultured in chondrogenic medium for 10 days before analyses. a Immunoblots showing the levels of Beclin1, LC3I, and LC3II in differentiating MSCs stimulated with varying intensities of LIPUS, with β-actin as the loading control. b Representative electron microscopy images showing autophagosomes (arrows); scale bars = 0.5 μm. c Left panel—representative immunofluorescence images showing LC3+ cells (green); scale bars = 50 μm. Right panel—bar graph comparing the number of LC3+ cells. The values are the mean ± SD of triplicate experiments; *P < 0.05

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