Fig. 1

PF14 delivers fluorescently labeled siRNA into hES cells with high efficiency. a Scheme of experiment: plating of cells (4.5 × 10⁵ cells/well) and start of transfection at 0 h. Transfection complexes remained in the growth medium for 24 h. During that period, 3 independent flow cytometry analyses were performed (at 4 h, 8 h, and 24 h). After 24 h, the medium was changed for one without complexes and flow cytometric analysis was performed at 32 h. siRNA was used at concentration of 30 nM. Symbols: red line—transfection, black triangle—plating cells and adding transfection complexes, gray triangle—flow cytometric analysis, white triangle—medium exchange. b Flow cytometric analysis of siRNA-Alexa Fluor 568 in hES cells at various time points. Cells incubated with siCtrl/PF14 complexes were used as controls. Bars and numbers on histogram indicate the percentage of transfected cells in siRNA-Alexa Fluor 568-treated sample. c Immunofluorescence analysis of siRNA-Alexa Fluor 568 (red) localization in siRNA/PF14-treated cells after 24-h incubation. Cell nuclei were stained with DAPI (blue) and actin cytoskeleton with phalloidin (green). Scale bar is 20 μm