Table 2 Primers used in polymerase chain reaction

B2M

Forward 5′-TGCTGTCTCCATGTTTGATGTATCT-3′

Reverse 5′-TCTCTGCTCCCCACCTCTAAGT-3′

BRA

Forward 5′-ATGACAATTGGTCCAGCCTT-3′

Reverse 5′-CGTTGCTCACAGACCACAG-3′

GATA4

Forward 5′-GCTCCTTCAGGCAGTGAGAG-3′

Reverse 5′-CTGTGCCCGTAGTGAGATGA-3′

HAND1

Forward 5′-TGCCTGAGAAAGAGAACCAG-3′

Reverse 5′-ATGGCAGGATGAACAAACAC-3′

NANOG

Forward 5′-CCTGTGATTTGTGGGCCTG-3′

Reverse 5′-GACAGTCTCCGTGTGAGGCAT-3′

OTX2

Forward 5′-GCTGGCTATTTGGAATTTAAAGG-3′

Reverse 5′-GGGTTTGGAGCAGTGGAAC-3′

PAX6

Forward 5′-AACAGACACAGCCCTCACAAAC-3′

Reverse 5′-CGGGAACTTGAACTGGAACTGAC-3′

POU5F1 (OCT4)

Forward 5′-CTGGAGCAAAACCCGGAGG-3′

Reverse 5′-CCTCAAAGCGGCAGATGGTC-3′

RPL13A

Forward 5′-CCTGGAGGAGAAGAGGAAAGAGA-3′

Reverse 5′-TTGAGGACCTCTGTGTATTTGTCAA-3′

SOX1

Forward 5′-CAGCAGTGTCGCTCCAATTCA-3′

Reverse 5′-GCCAAGCACCGAATTCACAG-3′

SOX2

Forward 5′-GTATCAGGAGTTGTCAAGGCAGAG-3′

Reverse 5′-TCCTAGTCTTAAAGAGGCAGCAAAC-3′

SOX17

Forward 5′-GGCGCAGCAGAATCCAGA-3′

Reverse 5′-CCACGACTTGCCCAGCAT-3′

TBP

Forward 5′-TGCACAGGAGCCAAGAGTGAA-3′

Reverse 5′-CACATCACAGCTCCCCACCA-3′