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Table 2 Primers used in polymerase chain reaction

From: Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14

Gene name Primer sequence
B2M Forward 5′-TGCTGTCTCCATGTTTGATGTATCT-3′
Reverse 5′-TCTCTGCTCCCCACCTCTAAGT-3′
BRA Forward 5′-ATGACAATTGGTCCAGCCTT-3′
Reverse 5′-CGTTGCTCACAGACCACAG-3′
GATA4 Forward 5′-GCTCCTTCAGGCAGTGAGAG-3′
Reverse 5′-CTGTGCCCGTAGTGAGATGA-3′
HAND1 Forward 5′-TGCCTGAGAAAGAGAACCAG-3′
Reverse 5′-ATGGCAGGATGAACAAACAC-3′
NANOG Forward 5′-CCTGTGATTTGTGGGCCTG-3′
Reverse 5′-GACAGTCTCCGTGTGAGGCAT-3′
OTX2 Forward 5′-GCTGGCTATTTGGAATTTAAAGG-3′
Reverse 5′-GGGTTTGGAGCAGTGGAAC-3′
PAX6 Forward 5′-AACAGACACAGCCCTCACAAAC-3′
Reverse 5′-CGGGAACTTGAACTGGAACTGAC-3′
POU5F1 (OCT4) Forward 5′-CTGGAGCAAAACCCGGAGG-3′
Reverse 5′-CCTCAAAGCGGCAGATGGTC-3′
RPL13A Forward 5′-CCTGGAGGAGAAGAGGAAAGAGA-3′
Reverse 5′-TTGAGGACCTCTGTGTATTTGTCAA-3′
SOX1 Forward 5′-CAGCAGTGTCGCTCCAATTCA-3′
Reverse 5′-GCCAAGCACCGAATTCACAG-3′
SOX2 Forward 5′-GTATCAGGAGTTGTCAAGGCAGAG-3′
Reverse 5′-TCCTAGTCTTAAAGAGGCAGCAAAC-3′
SOX17 Forward 5′-GGCGCAGCAGAATCCAGA-3′
Reverse 5′-CCACGACTTGCCCAGCAT-3′
TBP Forward 5′-TGCACAGGAGCCAAGAGTGAA-3′
Reverse 5′-CACATCACAGCTCCCCACCA-3′