Fig. 3

The 3′-UTR of GPNMB is a direct target of miR-508-5p. a Schematic illustration of the complementary sequence between miR-508-5p and the GPNMB 3′-UTR. The mutation sites on the 3′-UTR of GPNMB were underlined. b hDPCs were transfected with miR-508-5p mimics or siRNA, and the expression levels of GPNMB were determined by qRT-PCR on day 6. c The expression levels of GPNMB were detected by Western blotting after transfection of hDPCs on day 6. d The Western blot results of GPNMB protein were quantified. e Luciferase reporter assay data found that co-transfection of 293T cells with miR-508-5p and wild-type GPNMB 3′-UTR led to a decrease in luciferase activity. In contrast, there was no significant difference observed in the GPNMB-Mt group. *p < 0.05