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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: The suppressive effects of miR-508-5p on the odontogenic differentiation of human dental pulp stem cells by targeting glycoprotein non-metastatic melanomal protein B

Fig. 4

miR-508-5p inhibits the odontogenic differentiation of hDPCs. hDPCs were transfected with negative control (NC), negative control of siRNA (NC-Si), miR-508-5p mimics, or miR-508-5p siRNA, respectively. a On day 7 of odontogenic differentiation, the expression levels of the odontogenic marker genes (ALP, DMP-1, DSPP, and OCN) were detected by qRT-PCR. b The expression levels of the odontogenic marker genes (ALP, DMP-1, DSPP, and OCN) were detected by Western blotting on day 7 of odontogenic differentiation. c The Western blot results of GPNMB protein were quantified. d ALP activity decreased in the miR-508-5p mimics group and increased in the miR-508-5p siRNA on day 7 of odontogenic induction. e On day 14 after treatment, Alizarin Red S staining was performed to show the inhibitory effects of miR-508-5p mimics on the matrix mineralization of hDPCs. *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512