Fig. 1
From: MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway
Odontogenic differentiation of BMMSCs induced by TGC-CM. a BMMSCs showed colony formation after 48 h in culture. b BMMSCs reached 100% confluence after 7 days in culture. c Mineral deposits stained by Alizarin Red S in BMMSCs after osteogenic differentiation for 3 weeks. d Adipocytes stained by Oil Red O after adipo-induction for 3 weeks. e, f, g, and h The expression rate of CD11, CD90, CD29, and CD45. i Mineralization nodules and (j) ALP, DMP-1, and odontoblast-specific marker DSP protein levels of BMMSCs were significantly increased after incubated by TGC-CM for 7 and 14 days. Scale bar = 100 μm