Fig. 1

Characterization of exosomes that originated from U251 cells. a The different amount (5, 10, 20, 30, and 40 μg) of protein from U251 cell-derived exosomes were loaded and run on a 10% SDS gel and stained with Coomassie Blue. b The size of U251 cell-derived exosomes detected by Zetasizer Nano ZS. c Western blot analyses of exosomal markers (HSP70, Tsg101, and CD9). d, e Flow cytometry analyses of exosomal surface markers (CD63 and CD81)