Fig. 10

The levels of glycolysis pathway influenced by U251 cell-derived exosomes in hBMSCs. The hBMSCs were incubated with different concentrations (150, 300, and 600 μg/mL) of U251 cell-derived exosomes for 48 h. a–c The level of glucose consumption, lactate production, and cellular ATP production were assayed. U251 cell-derived exosomes could increase the glucose consumption and lactate and ATP production compared to the control group in the hBMSCs (*P < 0.05, **P < 0.01). d The expression levels of several key glycolytic pathway-related proteins (Glut-1, HK-2, and PKM-2) were measured by Western blotting after hBMSCs were treated with U251 cell-derived exosomes. e Bar graphs showing the relative protein expression level of Glut-1, HK-2, and PKM-2. The protein levels of GAPDH served as loading controls (*P < 0.05). f The mRNA expressions of Glut-1, HK-2, and PKM-2. GAPDH was used as the endogenous control (*P < 0.05)