Fig. 11

2-DG reversed the tumor-like phenotype transformation of hBMSCs under the treatment of U251 cell-derived exosomes. The hBMSCs were incubated with 300 μg/mL U251 cell-derived exosomes or 2.5 mmol/L 2-DG for 48 h. a 2-DG could suppress the proliferation of hBMSCs under the treatment of U251 cell-derived exosomes for 24 h, 48 h, and 72 h (*P < 0.05). b, c The effects of 2-DG on hBMSCs migration capability were assessed by the Transwell assay with or without Matrigel under the treatment of U251 cell-derived exosomes. The number of migrated or invaded cells was quantified by counting the number of cells from five random fields at a × 200 magnification (*P < 0.05). d, e Protein levels of MMP-2 and MMP-9 were examined by Western blotting under the treatment of U251 cell-derived exosome or 2.5 mmol/L 2-DG. GAPDH was an internal control (*P < 0.05)