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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis

Fig. 2

U251 cell-exosomes promote the proliferation of hBMSCs. a Cellular internalization of Dil-labeled U251 cell-derived exosomes into hBMSCs. b hBMSCs were treated with different concentrations of U251 cell-derived exosomes for different time points (24, 48, and 72 h), and then cell viability was measured using the CCK-8 analyses. The results showed U251 cell-derived exosomes could promote hBMSC proliferation in a time- and dose-dependent manner with respect to control cells (*P < 0.05). c Representative images of Ki67 immunostaining in hBMSCs treated with or without U251 cell-derived exosomes for 48 h. Green fluorescence indicated the Ki67-positive cells. d Protein expression of PCNA and C-myc was determined by Western blotting analysis in hBMSCs treated U251 cell-derived exosomes for 48 h. e Bar graphs showing the relative protein expression level of PCNA and C-myc in hBMSCs treated with U251 cell-derived exosomes for 48 h. The protein levels of GAPDH served as loading controls (*P < 0.05). f The hBMSCs exposed with exosomes for 48 h showed an increase in the S phase of the cell cycle compared to the controls. g Treatment of exosomes caused a S cell cycle arrest in a dose-dependent manner, the arresting rate increased more rapid in hBMSCs exposed with exosomes than control cells for 48 h (*P < 0.05, **P < 0.01). h Real-time PCR analysis of P21 and P16 mRNA expression in hBMSCs treated with U251 cell-derived exosomes for 48 h (*P < 0.05, **P < 0.01)

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