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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Microvesicles from human adipose stem cells promote wound healing by optimizing cellular functions via AKT and ERK signaling pathways

Fig. 5

Effect of ASC-MVs on the activation level of AKT and ERK signaling pathways. Western blot analysis of the phosphorylation level of AKT and ERK1/2 in cells treated with 20 μg/ml ASC-MVs for the indicated lengths of time. a The ratio of p-AKT/AKT in HUVECs was examined and included on the blots at different time points. b The ratio of p-ERK/ERK in HUVECs was determined. d The ratio of p-ERK/ERK and p-AKT/AKT in HaCAT was determined. e The ratio of p-ERK/ERK and p-AKT/AKT in fibroblasts was determined. Qualification of the ratio of p-ERK/ERK and p-AKT/AKT at different time points. c, f Qualified data shown in a and b. g, h Qualified data shown in d. i, j Qualified data shown in e. N = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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