Fig. 6

Flow cytometric analysis of indicated activating and inhibiting receptors by NK cells harvested from NK/pMSC cytolytic experiments. As compared with untreated NK cells, pMSCs significantly decreased NK cell expression of CD69 (a), NKG2D (b), and CD94 (c) at all NK to pMSC ratios (25:1, 50:1, and 100:1), *P < 0.05; significantly decreased NK cell expression of NKp30 at 25:1 and 50:1 NK to pMSC ratios, *P < 0.05 (d); significantly decreased NK cell expression of NKp46 at 100:1 NK to pMSC ratio, *P < 0.05 (e); significantly increased NK cell expression of DNAM (CD226) at all indicated NK to pMSC ratios, *P < 0.05 (f); and significantly increased NK cell expression of NKp30 at 25:1 NK to pMSC ratio, *P < 0.05 (d). pMSCs had no significant effect on NK cell expression of NKp44, P > 0.05 (g). Experiments were carried out in triplicate and repeated for ten times using ten independent NK/pMSC cytolytic experiments where in each experiment NK cells and pMSC were prepared from the peripheral blood of ten different healthy subjects and ten different normal human term placentae, respectively. Bars represent standard errors