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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Preconditioning human natural killer cells with chorionic villous mesenchymal stem cells stimulates their expression of inflammatory and anti-tumor molecules

Fig. 8

NK cell secretion of cytokines in the NK/pMSC cytolytic experiment where IL2-stimulated NK cells lysed pMSCs at 25:1, 50:1, and 100:1 NK to pMSC ratios. NK cell secretion of IL1ra, IL1β, IL10, IL12, IFNγ, GM-CSF, and MIF was evaluated using sandwich ELISA. Cytokine secretion profiles showed that pMSCs in the cytolytic experiment significantly increased the secretion of IL1ra (a), *P < 0.05, and significantly decreased the secretions of IL1β (b), IL-12 (c), IFNγ (d), GM-CSF (e), MIF (f), and IL10 (g), *P < 0.05, by NK cells after 24 h culture of IL-2-activated NK cell co-cultured with pMSCs as compared to IL-2-stimulated NK cells cultured alone (no pMSCs). Each experiment was carried out in duplicate using culture medium obtained from ten independent NK/pMSC cytolytic experiments where in each experiment NK cells and pMSC prepared from the peripheral blood of ten different healthy subjects and ten different normal human term placentae, respectively. Bars represent standard errors

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Stem Cell Research & Therapy

ISSN: 1757-6512