Fig. 2
Differentially expressed miRNA profiling and bioinformatic analysis. Differentially expressed miRNAs (P < 0.05) were analyzed by hierarchical clustering of log2 values. a Venn diagram showing separate and overlapping differential expression of miRNAs during iPSCs-derived IPCs at the early, middle, and late stages of step 3 compared to that of iPSCs. b Heatmap shows selected differentially expressed miRNAs (fold change 1.5 and P value < 0.05). c The regulatory network of miRNA-target genes. Green circles represent target genes, and purple circles represent differentially expressed miRNAs. d Differentially expressed pathways were analyzed by gene ontology (GO) analysis. e KEGG pathway enrichment analysis for target genes. The size of the bubbles represents the number of target genes associated with each pathway