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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9

Fig. 3

Overexpression of miR-690 inhibits pancreatic differentiation potential. This group of experiments tested the functions of iPSCs-derived IPCs on day 4 of the second step. Quantitative RT-PCR analysis of the expression levels of a miR-690 and b several key transcription factors during the development of pancreatic β-cells (Pdx1, NGN3, Nkx2.2, Nkx6.1, Gata4, Gata6, Pax4, Pax6). GAPDH was used as the internal control. Error bars show mean ± standard deviation (SD) (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Immunofluorescence assay (Nkx6.1 and Pdx1, green; nuclear, blue; scale bar 75 μm) for protein expression level of Nkx6.1 and Pdx1

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